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Here is a short tutorial:

For example, if you want to get sequences upstream of genes coding for adenine transporters:
First select the checkboxes for:

After, choose the condition to which you want to limit your search:

Please select a field:

Field Explanation or Example

cdd Conserved domains

nickel ABC transporter, nickel/metallophore periplasmic binding protein

cds Coding Sequences

gene name like rhlA
product name like Mg transporter
start position
end position
strand direction

fragment Chromosome information

RefSeq accession number like NC_000913
strain information like Newman
bacteria; elusimicrobia; environmental samples
Staphylococcus aureus subsp. aureus str. Newman

gap3 Sequence information for 3 prime-UTR

start position of 3 prime-UTR
end position of 3 prime-UTR
strand direction of 3 prime-UTR
sequence of 3 prime-UTR in the same strand as the gene

gap5 Sequence information for 5 prime-UTR

start position of 5 prime-UTR
end position of 5 prime-UTR
strand direction of 5 prime-UTR
sequence of 5 prime-UTR in the same strand as the gene

operon Operon

glutamine synthetase, type I-->nitrogen regulatory protein P-II

organism Organism

spiral/rod/coccus etc
Non-halophilic/halophilic/Moderate halophilic
Q fever

rna_family Family of RNA according to Rfam

cis-reg; riboswitch;

rna_known Known RNA according to Rfam

start position of RNA
end position of RNA
strand of RNA




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